CUSTOM ITEM: HPLC Column, Diamond Hydride 2.o, 2.2um, 2.1mm ID x 150mm Length, 120A. Packed in a "metal free" Stainless Steel Column and Frits. Cogent TYPE-C Brand. 1 EA.
Additional Info:
High‑Resolution Bio‑Inert Diamond Hydride™ Performance for Demanding LC‑MS Methods
The 70200‑15D‑2 Bio‑Inert Diamond Hydride™ HPLC Column is a custom‑manufactured, high‑efficiency analytical column designed for laboratories requiring maximum resolution, metal‑free flow paths, and stable low‑flow performance. Packed with 2.2 µm Diamond Hydride™ stationary phase using Cogent TYPE‑C™ silica hydride technology, and housed in bio‑inert, metal‑free stainless‑steel column hardware and frits, this column delivers rapid equilibration, consistent retention, and long service life for highly polar and biologically sensitive analytes.
The 2.1 mm ID × 150 mm length configuration is selected when maximum separation efficiency and peak capacity are required in HILIC‑based LC‑MS workflows.
Why the 2.1 mm × 150 mm Configuration Is Selected
This format is intentionally chosen for methods where resolution and analytical confidence outweigh speed:
Increased plate count for separation of closely eluting polar compounds
Improved quantitative reliability in complex matrices
Narrow‑bore geometry enhances mass sensitivity in LC‑MS
Bio‑inert hardware minimizes analyte loss and metal‑induced artifacts
The combination of longer column length and 2.2 µm particles provides substantially higher resolving power compared to short LC‑MS columns, making this format suitable for confirmatory, validated, and regulated methods.
Diamond Hydride™ Stationary Phase Advantage
Built on Cogent TYPE‑C™ silica hydride technology, the Diamond Hydride™ stationary phase offers fundamental advantages over conventional bonded‑silica HILIC materials:
Rapid equilibration, even with high organic mobile phases
Highly stable and reproducible retention mechanisms
Mixed‑mode interaction capability for difficult or weakly retained analytes
Long usable column lifetime under routine and regulated conditions
These attributes support consistent performance across extended analytical sequences commonly encountered in LC‑MS laboratories.
Ideal Applications
The 70200‑15D‑2 bio‑inert Diamond Hydride column is routinely employed for:
Small, highly polar molecules
Amino acids and organic acids
Polar peptides and metabolites
Proteins and other metal‑sensitive biological analytes
It is particularly valuable where trace‑level accuracy, resolution, and reproducibility are critical.
High‑Resolution LC‑MS and UV Detection
The 150 mm length provides enhanced separation capacity for complex samples, making this column well‑suited for:
LC‑MS methods requiring high resolution and confidence in compound identification
Applications where shorter columns do not adequately resolve closely eluting analytes
Methods supporting validation, robustness testing, and long analytical runs
For UV detection, careful attention to low system volume is recommended to maintain peak integrity with narrow‑bore columns.
LC‑MS Compatibility and System Considerations
This column is fully LC‑MS compatible and commonly used in HILIC‑based MS workflows. Because of the 2.1 mm internal diameter, 150 mm length, and 2.2 µm particle size, the system must support:
Accurate and stable low‑flow operation
Optimized plumbing with minimized dead volume
Standard HPLC systems that cannot reliably deliver low flow rates may not be suitable.
Operating Guidelines and System Requirements
Typical flow‑rate range: ~0.2–0.3 mL/min (method‑ and solvent‑dependent)
System compatibility: HPLC or UHPLC systems capable of precise low‑flow delivery
Special requirements: Low‑volume flow cell and optimized connections recommended
Flow rates can be adjusted to balance resolution, pressure limits, and detector sensitivity.
Custom Item Notice
This product is classified as a Custom Item. As with all Diamond Hydride™ bio‑inert, prep, and semi‑prep columns, this item can be made to order, non‑returnable.